Endocrine Abstracts

Aims This is an investigation of potential oxidative effects of AGE in response to the antioxidant glutathione in cultured human gingival fibroblasts, using radiolabelled steroids as substrates and their product 5 alpha dihydrotestosterone (DHT) as a marker of wound healing.Methods Confluent monolayer cultures of human gingival fibroblasts were incubated with 14C-Testosterone and 14C-4-androstenedione respect...

Aims Nicotine (N) has detrimental effects on fibroblasts compounding those of AGE in uncontrolled diabetes. The impact of the antioxidant glutathione (G) is investigated to clarify mechanisms involved, using 5 alpha dihydrotestosterone (DHT) as a hormone marker of healing.MethodsHuman gingival fibroblasts in confluent culture were incubated in Eagle’s MEM, with 14C-testosterone / 14C-4-androstenedione an...

Much less information is available on the thyroid hormones at different age groups, laying, non-laying and methimazole (MMI) treated japanese quails. All birds were kept under 14:10 hour light schedule and were provided feed and water adlibitum. Fifteen japanese quailsin each age groups, laying, non-laying and MMI treated were decapitated for collection of plasma. Thyroid hormones (T3 and T4) were estimated using ELISA commercial kits. The results showed that plasma thyroid ho...

Eight healthy pregnant goats, aged 2-4 years, were selected for this study. Blood samples were obtained in tubes containing EDTA at different stages of pregnancy, at parturition and on days 1, 7 and 14 postpartum. Blood from foeti was also collected on days 1 and 7 after their birth. Plasma was separated, frozen at -20 celsius till analysed for thyroid hormones (T3 and T4) using ELISA comercial kits. Thyroxine (T4) concentration was higher during prepartum, decreased significa...